PREVENTION OF H-AGGREGATES FORMATION IN CY5 LABELED MACROMOLECULES

Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules

H-aggregates of the cyanine dye Cy5 are formed during covalent linkage to the cationic macromolecule Poly(allylamine) (PAH).The nonfluorescent H-aggregates strongly restrict the usage of the dye for analytical purposes and prevent a quantitative determination of the labeled macromolecules.The behavior of the H-aggregates has been studied by investi

read more

Nitrogenous compounds characterized in the deterrent skin extract of migratory adult sea lamprey from the Great Lakes region.

The sea lamprey (Petromzons marinus) is a devastating invasive species that represents a significant impediment to restoration of the Laurentian Great Lakes.There is substantial interest in developing environmentally benign control strategies for sea lamprey, and many other aquatic invasive species, that employ the manipulation of semiochemical inf

read more


Meta-analysis of six dairy cattle breeds reveals biologically relevant candidate genes for mastitis resistance

Abstract Background Mastitis is a disease that incurs significant costs in the dairy industry.A promising Headwear approach to mitigate its negative effects is to genetically improve the resistance of dairy cattle to mastitis.A meta-analysis of genome-wide association studies (GWAS) across multiple breeds for clinical mastitis (CM) and its indicato

read more

Centering accessibility, increasing capacity, and fostering innovation in the development of international eDNA standards

Environmental DNA (eDNA) includes a set of rapidly emerging technologies that have the potential to support environmental monitoring and biodiversity conservation through novel, non-invasive, cost-effective and democratic methods and tools.Meanwhile, Cold Cup eDNA researchers are developing international standards for eDNA technologies, methods and

read more